Jun-Geon Je, Jimin Hyun, Sang-Woon Lee, and You-Jin Jeon*
Department of Marine Life Science, Jeju National University, Jeju 63243, Korea
*Corresponding author (You-Jin Jeon): Tel: +82-64-754-3475; Fax: +82-64-756-3493; E-mail: youjin@jejunu.ac.kr
In this study, we purified and structurally characterized sulfated polysaccharides from brown algae, Sargassum binderi (S. binderi, SBPs), and evaluated their biological activity in vitro and in vivo. SBPs, Fraction 4 (SBP-F4) by DEAE, with an average molecular weight of 2.867× 104 g/mol, had the highest polysaccharide and sulfate content (75.15±0.25% and 24.08±0.18%, respectively). The biological activities of SBP-F4 were investigated further in vitro and in vivo. Our results showed that SBP-F4 significantly suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in LPS-activated macrophages. Moreover, in the LPS-treated zebrafish model, a significant decrease in cell death and NO production was observed. Collectively, these results showed that SBPs not only exert protective effects against LPS-induced cytotoxicity but also inhibit macrophage activation and their anti-inflammatory activity. Therefore, polysaccharides derived from S. binderi are potential anti-inflammatory agents for use in clinical settings.
Keywords: Sargassum binderi, Enzyme hydrolysate, sulfated polysaccharide, Anti-inflammatory