Noer Kasanah1, Maria Ulfah1, Susilawati1, Triyanto1
1Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia
Aquaculture is an important industry in supporting food security. Vibriosis is one of the problem in aquaculture industry that cause economic loss. Increasing incidence of vibriosis and escalating cases of resistance Vibrio spp to antibiotics have driven the searching for new antivibrio compounds. Red seaweed (Rhodophyta) is a source of secondary metabolites that have a broad spectrum of biological activity, and contain rich bioactive components that play a role in inhibiting bacterial growth. The objectice of our reseach to determine anti vibrio compounds and the molecular taxonomy of potential red seaweed from Gunungkidul, Indonesia. Extracts ethanolic were tested against Vibrio parahaemolyticus. The bioactivity was examined by in situ bioautography test and microplate assay using resazurin as an indicator. Bioactive compounds were detected using GC-MS and LC-MS. Dereplication of compounds was checked using database marine natural products. Molecular identification of seaweed was carried out based on amplification of the CO1 gene. Results showed that the potential red seaweed was identified as Laurencia saitoi with potential activity against V. parahaemolyticus at 0.078 µg/ml. The bioactive compounds were identified as aristolene and other new metabolites.
Key words : Red seaweed, Vibrio parahaemolyticus, Laurencia saitoi, aristolene