Jong-il Choi
Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 61186, Republic of Korea
Recently, mutant strain P. yezoensis Py2K was generated by gamma irradiation. The strain exhibited enhanced growth and high-temperature tolerance when compared to the wild type. But, the analysis of mutant Py2K was not investigated at molecular level. To counter this problem, genome-wide transcriptome profiling and de novo assembly has been extensively employed to investigate multiple characteristics of this species. With the aim of studying the molecular characteristics of Py2K, we performed transcriptome sequencing using RNA-Seq. Based on those data, genes that are differentially expressed can be identified to help to determine the mechanism responsible for the Py500G phenotype. After sequencing, De novo transcriptome assembly and redundancy removal generated 167,165 transcripts. The assembly were annotated in NCBI nr, Swiss-Prot, Pfam, KEGG, GO and KOG databases. To unravel the differences in Py2K and wild-type (PyWT), we mapped Py2K and PyWT reads to the assembly and calculated the expression levels. In total, there were 15,979 transcripts that were differentially expressed. Among the differentially expressed transcripts, candidate genes were identified with well-known growth and development functions.